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MicroRNAs (miRNAs) are newly discovered class of non-coding small RNAs of 20-24 nucleotide length that regulate gene and protein expression in plants and animals. MiRNAs have so far been identified mostly by specific cloning of small RNA molecules, complemented by computational methods.

Computational identification of miRNA is faster and generally follows AB initio and sequence alignment methods. Here we developed MIRPI, a new software that finds highly conserved miRNAs from the given gene sequences by using non-repeated miRNAs of the users interest.

The software is designed to identify the required miRNA’s, their targets and also to calculate the Adjusted Minimum Folding free Energy (AMFE) and Minimum Folding free Energy Index (MFEI) which are one of the most important parameters to confirm the miRNAs. MIRPI is developed by PERL (Practical Extraction and Report Language) and Tk (Tool kit widgets). It is a user friendly, portable offline software that works in all windows OS of 3 MB size.