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Geneious PRO x64
Geneious PRO x64 6.0.4   
... features sequence alignment and phylogenetic analysis, contig assembly, primer design and cloning, access to NCBI and UniProt, ... Cloning, restriction mapping, digestion and ligation * Automated primer design with degeneracy support * Keep a primer ...
Trialware | $249.00

Unipro UGENE 64-bit
Unipro UGENE 64-bit 50.0   
... source of HMMER 2 and HMMER 3; PCR Primers design using Primer 3; Cloning in silico capabilities; Queries to NCBI ...
Freeware
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