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The DynaFit application was developed to perform nonlinear least-squares regression of chemical kinetic, enzyme kinetic, or ligand-receptor binding data. The experimental data can be either initial reaction velocities in dependence on the concentration of varied species (e.g., inhibitor concentration vs. velocity), or the reaction progress curves (e.g., time vs. absorbance).

The main advantage in using the program DynaFit is in the ability to characterize the (bio)chemical reacting system in terms of symbolic, or stoichiometric, equations. For example, the ``slow, tight'' inhibition of a dissociative dimeric enzyme is described by the following text:

Monomer + Monomer Enzyme : k1 k2
Enzyme + Inhibitor Complex : k3 k4
Enzyme + Substrate ReactiveX : k5 k6
ReactiveX -- Product + Enzyme : k7 k8

The names of chemical species ("Monomer", "Enzyme", etc.) are entirely arbitrary and can be freely chosen by the investigator.